Regulatory ubiquitylation in response to DNA double-strand breaks |
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| Authors: | Stephanie Panier Daniel Durocher |
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| Institution: | 1. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5;2. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada;1. Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, India;2. Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India;3. Department of Pulmonary Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India;1. Biological Science, Graduate School of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8529, Japan;2. Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan;1. Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA, USA;1. Obstetrics and Gynaecology, University of Melbourne and the Royal Women''s Hospital, Parkville 3052, VIC, Australia;1. Institute of Molecular Cancer Research, University of Zurich, Zurich 8057, Switzerland;2. Unidad de Investigación, Hospital Universitario de Canarias, Instituto de Tecnologías Biomédicas, Ofra s/n, La Cuesta, La Laguna, Tenerife 38320, Spain |
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| Abstract: | DNA double-strand breaks (DSBs) are highly cytolethal DNA lesions. In response to DSBs, cells initiate a complex response that minimizes their deleterious impact on cellular and organismal physiology. In this review, we discuss the discovery of a regulatory ubiquitylation system that modifies the chromatin that surrounds DNA lesions. This pathway is under the control of RNF8 and RNF168, two E3 ubiquitin ligases that cooperate with UBC13 to promote the relocalization of 53BP1 and BRCA1 to sites of DNA damage. RNF8 and RNF168 orchestrate the recruitment of DNA damage response proteins by catalyzing the ubiquitylation of H2A-type histones and the formation of K63-linked ubiquitin chains on damaged chromatin. Finally, we identify some unresolved issues raised by the discovery of this pathway and discuss the implications of DNA damage-induced ubiquitylation in human disease and development. |
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