Alkyl acyl ethanolamine glycerophosphatides in rat intestinal mucosa

The ethanolamine glycerophosphatides (EGP) were isolated from rat mucosal lipids by column and thin-layer chromatography and were found to contain alkyl acyl EGP. After hydrolysis and acetylation, the resulting alkyl diacetyl glycerols were separated by gas-liquid chromatography and the paraffinic moieties were shown to consist of C18- and C20-saturated and C18-monounsaturated residues. The fatty acid compositions of the 1,2-diacyl glycerols and 1-alkyl-2 acyl glycerols obtained by treatment of the phosphatides with phospholipase C were determined. The 1-alkyl 2-acyl glycerols were rich in polyunsaturated acyl residues.