| 银杏产黄酮内生真菌的分离与鉴定 |
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| 引用本文： | 韩晓丽,康冀川,何劲,张禧庆,黄庆华. 银杏产黄酮内生真菌的分离与鉴定[J]. 菌物研究, 2008, 6(1): 40-45 |
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| 作者姓名： | 韩晓丽 康冀川 何劲 张禧庆 黄庆华 |
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| 作者单位： | 贵州省农业生物工程重点实验室,贵阳,550025;贵州省农业生物工程重点实验室,贵阳,550025;贵州省农业生物工程重点实验室,贵阳,550025;贵州省农业生物工程重点实验室,贵阳,550025 |
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| 基金项目： | 财政部产业技术成果转化项目 |
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| 摘 要： | 为实现真菌发酵生产黄酮,用HPLC和氯化铝比色法,从四川、贵州、山东等地采集的银杏根、茎、叶中分离到7株产黄酮真菌。分子和形态学鉴定结果表明:这些菌株分别属于Colletotrichum和Fusarium,菌株QC102经鉴定为Colletotrichumacutatum Simmonds,其总黄酮产量为8.2μg/mL。
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| 关 键 词： | 银杏 内生真菌 黄酮 槲皮素 HPLC |
| 文章编号： | 1672-3538(2008)01-0040-06 |
| 修稿时间： | 2007-10-22 |
Isolation and Identification of Endophytic Fungi of Falvonoid-Producing Ginkgo biloba |
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| HAN Xiao-li,FANG Ji-chuan,HE Jin,ZHANG Xi-qing,HUANG Qing-hua. Isolation and Identification of Endophytic Fungi of Falvonoid-Producing Ginkgo biloba[J]. Journal of Fungal Research, 2008, 6(1): 40-45 |
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| Authors: | HAN Xiao-li FANG Ji-chuan HE Jin ZHANG Xi-qing HUANG Qing-hua |
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| Affiliation: | HAN Xiao-li, KANG Ji-chuan , HE Jin, ZHANG Xi-qing, HUANG Qing-hua (1. Guizhou Key Laboratory of Agricultural Biotechnology , Guiyang 550025, China ; 2. Biochemical Engineering Center of Guizhou Province, Guiyang 550025, China ) |
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| Abstract: | Seven fungal isolates were found to produce falvonoids which were acquired from root, stem and leaves of Ginkgo biloba L. collected in the areas of Sichuan, Guizhou and Shandong provinces in Chi- na by means of HPLC and AlCl3 chromatometry. The further molecular and morphological identification indicated that these fungal isolates belonged to Colletotrichum and Fusarium respectively. The isolate QC102 was indentified as C. acutatum Simmonds which can produce falvonoids up to 8.2μg/mL. |
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| Keywords: | Ginkgo biloba endophytic fungi falvonoids quercetin HPLC |
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