Antigenic stimulation of lymphocytes. II. Immunogenicity of dinitrophenyl-polyethylene oxide antigens. |
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| Authors: | J S Peacock T Krakauer L E Perryman H Krakauer |
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| Institution: | 1. The Program in Biochemistry and Biophysics of the Department of Chemistry, Washington State University, Pullman, Washington 99164 U.S.A.;2. The Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 U.S.A. |
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| Abstract: | Synthetic antigens consisting of dinitrophenyl groups attached to linear or branched-chain polyethylene oxide stimulate anti-DNP antibody production in rabbits and a proliferative response in vitro in immune rabbit lymphocytes. A requirement for immunogenicity is divalence. Linear DNP2PEO is most effective at a molecular weight of 104, but a clear response is obtained even at 103. The optimal valence of DNPnPEO (n = 4–80, MW, 4 × 105) is 20. Destruction of T cells with antithymocyte serum does not impair the in vitro response of the remaining B lymphocytes to DNP2PEO, indicating that these antigens are T independent. |
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