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The ras-related ral gene maps to chromosome 7p15-22 |
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| Authors: | Marie-Françoise Rousseau-Merck Alain Bernheim Pierre Chardin Roger Miglierina Armand Tavitian Roland Berger |
| Institution: | (1) Unité INSERM U 301 et Laboratoire d'Oncologie et d'Immunohématologie, L.O.I., LP 101, CNRS, 1, avenue Claude Vellefaux, F-75475 Paris Cédex 10, France;(2) Unité INSERM U 248, Faculté de Médecine Lariboisière-Saint Louis, Hopital Saint Louis, 1, avenue Claude Vellefaux, F-75475 Paris Cédex 10, France;(3) Service Commun de Tri Cellulaire, Institut de Recherches sur les Maladies du Sang, Hopital Saint Louis, 1, avenue Claude Vellefaux, F-75475 Paris Cédex 10, France;(4) Laboratoire de Cytogénétique, U301, Centre G. Hayem, Hôpital, Saint Louis, 1, avenue Claude Vellefaux, F-75475 Paris Cédex 10, France |
| Abstract: | Summary Human cDNAs coding for the new protein ral that shares 50% homology with the ras proteins have been recently isolated. A 600-bp fragment carrying mainly the coding region was used to localize the ral gene by hybridization with sorted chromosomes and in situ hybridization. Direct molecular hybridization on sorted chromosomes using a single laser illumination allowed the assignment of the ral gene to a region of the flow karyotype containing chromosomes 7, 8 and X. With dual laser analysis ral was assigned to the fraction containing chromosome 7. In the 331 human metaphases hybridized with the 3H-labelled insert, the silver grain distribution showed a unique major signal on chromosome 7p15-22. |
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