Facile synthesis of L-malic acid by a consecutive enzymatic reaction |
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| Authors: | Shui-Tein Chen Shui-Chyr Duh Kung-Tsung Wang |
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| Affiliation: | (1) Laboratory of Biocatalyst, Institute of Biological Chemistry, Academia Sinica, P.O. Box 23-106, 10098 Taipei, Taiwan;(2) Institute of Biochemical Science, National Taiwan University, Taipei, Taiwan;(3) Department of Chemistry, National Taiwan University, Taipei, Taiwan |
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| Abstract: | Procedures for a consecutive reaction catalyzed by fumarase and followed by aspartase in ammonium fumarate buffer to facilitate the separation of product have been developed. Fumarate was converted to L-malate in 83%, and the rest of the starting material was converted to aspartate in 16.7% to make a total conversion of 99.7%. After the solvent was evaporated, the malic acid was dissolved in acetone, and the aspartic acid was insoluble. A simple filtration could separate both products. |
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