Cotyledonary responses to maternal selenium and dietary restriction may influence alterations in fetal weight and fetal liver glycogen in sheep |
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| Authors: | LA Lekatz MA Ward PP Borowicz JB Taylor DA Redmer AT Grazul-Bilska LP Reynolds JS Caton KA Vonnahme |
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| Institution: | 1. Center for Nutrition and Pregnancy, Department of Animal Sciences, North Dakota State University, Fargo, United States;2. USDA-ARS, U.S. Sheep Experiment Station, Dubois, ID, United States;1. Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Zacatecas, Carretera Panamericana Zacatecas-Fresnillo Km 31.5, 98500 El Cordovel Enrique Estrada, Zacatecas, Mexico;2. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, 04510 México, DF, Mexico;3. United States Department of Agriculture, 11940 Don Haskins Dr. Suite E-3, El Paso, TX 79936, USA;4. Departamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Henry Dunant s/n Zona Pronaf, 32315 Ciudad Juárez, Chihuahua, Mexico;1. INDEGSAL, University of León, 24071 León, Spain;2. Department of Molecular Biology, University of León, 24071 León, Spain;3. Department of Medicine, Surgery and Veterinary Anatomy, University of León, 24071 León, Spain;4. DEPI, Institute of Technology of Conkal, Conkal, Mexico;1. Department of Animal Science, University of Wyoming, Laramie, WY, 82071, United States;2. Center for the Study of Fetal Programming, Department of Animal Science, University of Wyoming, Laramie, WY, 82071, United States;1. Department of Animal Reproduction, Faculdade de Ciências Agrárias e Veterinárias – UNESP, Av. Prof. Paulo Donato Castellane S/N, 14884-900, Jaboticabal, SP, Brazil;2. Department of Veterinary Clinical and Surgery, Faculdade de Ciências Agrárias e Veterinárias – UNESP, Av. Prof. Paulo Donato Castellane S/N, 14884-900, Jaboticabal, SP, Brazil;3. Universidade Federal dos Vales do Jequitinhonha e Mucuri, UFVJM, Avenida Vereador João Narciso 1380, 38610-000, Unaí, MG, Brazil;4. Veterinary Medicine College, Universidade Federal do Pará, Av. dos Universitários - Jaderlândia, 68746-360, Castanhal, PA, Brazil;5. Universidade Federal do Recôncavo da Bahia, Rua Rui Barbosa 710, 44380-000, Cruz das Almas, BA, Brazil |
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| Abstract: | To examine the effects of maternal supranutritional selenium (Se) and nutrient restriction during mid and late gestation on placental characteristics and fetal liver glycogen, ewes received either adequate Se (ASe) or high Se (HSe) prior to breeding. On d 64 of gestation, ASe and HSe ewes remained at 100% of requirements (controls; CON) or were restricted (RES; 60% of requirements). On d 135 of gestation, fetal weight (P ≤ 0.08) was greatest in both HSe and CON ewes. Placentome number, mass, and caruncular and cotyledonary weight were not different (P ≥ 0.17) among treatments. Fetal mass:placental mass ratio was less (P = 0.06) in RES compared to CON ewes. Compared to ASe, HSe exhibited increased (P ≤ 0.08) cellular proliferation and DNA concentration and decreased (P = 0.07) cellular size in cotyledonary tissue. Nutritional restriction decreased (P ≤ 0.08) cotyledonary protein concentration and cellular size. VEGF receptor 1 (Flt) mRNA in cotyledonary tissue was greater in HSe compared with ASe ewes (P = 0.06) and in RES compared with CON ewes (P = 0.08). There was no effect of diet on caruncular growth variables (P ≥ 0.13) or on placental vascularity (P ≥ 0.11). Progesterone was greater (P ≤ 0.08) in ASe–RES ewes compared to all groups at d 90 and ASe–CON and HSe–CON at d 104. Although fetal glucose and cortisol concentrations were not affected by diet, fetal liver glycogen was greater (P = 0.04) in ASe–RES compared to ASe–CON and HSe–RES ewes with HSe–CON being intermediate. Both Se and nutritional plane may impact placental function and fetal growth, as fetal weight and liver glycogen are altered despite similar placental vascularity measurements. |
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