Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes |
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| Authors: | Mithat Evecen Ümüt Cirit Kamber Demir Özen Banu Özda? Muzaffer Ta? Sema Birler Serhat Pabuccuo?lu |
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| Institution: | 1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey;2. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dicle University, 21280 Diyarbakir, Turkey;1. Department of Animal Science, Division of Applied Life Science, Gyeongsang National University, Jinju, Gyeongsangnam-do, Republic of Korea;2. Animal Biotechnology Division, National Institute of Animal Science, Suwon Gyeonggi-do, Republic of Korea;3. Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi-do, Republic of Korea;4. Animal, Dairy, and Veterinary Sciences Department, Utah State University, Logan, Utah, USA;5. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongsangnam-do, Republic of Korea;1. Department of Animal Reproduction, INIA, Madrid, Spain;2. Department of Poultry Production, Faculty of Agriculture, Assiut University, Asyut, Egypt;3. Department of Animal Breeding, INIA, Madrid, Spain;1. Laboratory of Animal Reproduction, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile;2. Laboratory of Animal Physiology, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile;1. Laboratory of Animal Reproduction, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile;2. Laboratory of Animal Physiology, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile;1. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey;2. Department of Biostatistics, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey;3. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey |
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| Abstract: | Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The present study investigated the effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. The donor bitches were categorized into three groups based on stage of estrus cycle: follicular (proestrus or estrous), luteal (diestrus) and anestrus. One ovary of each pair collected from 39 mature bitches was transported in Phosphate Buffer Saline (PBS) at 4 °C while the other was transported at 37 °C. A total of 1138 Grade I COCs obtained from all ovaries were grouped and matured in modified synthetic oviduct fluid (mSOF) supplemented with follicle stimulating hormone (FSH), luteinizing hormone (LH), essential and non-essential amino acids at 38.5 °C in a humidified 5% CO2, 5% O2, and 90% N2 atmosphere for 72 h. The nuclear maturation rates were evaluated by aceto–orcein staining.Oocytes harvested from follicular and luteal ovaries have a significantly higher maturation rates (MI + MII) than the oocytes from anestrual ovaries in the 37 °C group (p < 0.05). However, oocytes harvested from anestrual ovaries transported at 4 °C had the highest maturation (MI + MII) rate, and the difference between anestrual and luteal ovary groups was significant (p < 0.05). The oocytes from anestrual ovaries transported at 4 °C have significantly higher maturation rates than those transported at 37 °C (p < 0.0001). However, the transport temperature (37 or 4 °C) did not significantly affect the maturation (MI + MII) rates of oocytes harvested from the luteal (p = 0.61) and follicular (p = 0.48) stage ovaries.It can be concluded from this study that (1) both transport temperature and transport temperature × estrus cycle stage interaction effected the maturation rates, while estrus cycle stage alone did not, and (2) transporting canine ovaries at 4 °C can improve in vitro maturation rates in oocytes harvested from anestrous ovaries. |
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