Effect of follicular wave synchronization on in vitro embryo production in heifers |
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| Authors: | A.F. Ramos R. Rumpf J.U. Câmara M.R. Mollo I. Pivato A.P. Marques R. Sartori |
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| Affiliation: | 1. Veterinary School of Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;2. Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil;3. Cidasc, Indaial, SC, Brazil;4. Department of Animal Science, ESALQ, University of São Paulo (USP), Piracicaba, SP, Brazil;1. Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;2. Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;1. Department of Preventive Veterinary Medicine and Animal Reproduction, FCAV-UNESP, Jaboticabal, Brazil;2. Vida Reprodutiva Consultoria, Cravinhos, Brazil;3. Department of Surgery, FMVZ-USP, Pirassununga, Brazil;4. Department of Genetics and Evolution, CCBS-UFSCar, São Carlos, Brazil;5. Department of Animal Nutrition and Production, FMVZ-USP, Pirassununga, Brazil;6. Department of Animal Reproduction, FMVZ-USP, São Paulo, Brazil;7. Department of Veterinary Medicine, FZEA-USP, Pirassununga, Brazil;8. Department of Biostatistics, IB-UNESP, Botucatu, Brazil;9. Vitrogen, Cravinhos, Brazil;2. Department of Veterinary Medicine, Departamento de Medicina Veterinaria (DMV), Universidade Federal de Lavras, 37200-000, Lavras, MG, Brazil;3. EMBRAPA Gado de Leite, 36038-330 Juiz de Fora, MG, Brazil;1. Vida Reprodutiva Consultoria S/S LTDA, Cravinhos, SP, Brazil;2. Department of Animal Reproduction, FMVZ-USP, São Paulo, SP, Brazil;3. Vitrogen, Cravinhos, SP, Brazil;4. Department of Animal Reproduction, FCAV–UNESP, Jaboticabal, SP, Brazil;1. Instituto de Reproducción Animal Córdoba (IRAC), General Paz, PC.5145, Córdoba, Argentina;2. Instituto de Ciencias Básicas, Carrera de Medicina Veterinaria, Universidad Nacional de Villa María, Villa del Rosario, PC.5963, Córdoba, Argentina;3. Recombinetics Inc., Saint Paul, MN, USA;4. Laboratório de Biotecnologías de la Reproducción Animal, Escuela Superior Politécnica Agropecuaria de Manabí (ESPAM), Calceta, Manabí, Ecuador;1. Universidade Federal do Pampa (UNIPAMPA), Lab Biotecnologia da Reprodução (BIOTECH), 97.500-970, Uruguaiana, RS, Brazil;2. Zoetis Indústria de Produtos Veterinários Ltda, São Paulo, São Paulo, Brazil |
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| Abstract: | Aiming to achieve the ideal time of ovum pick-up (OPU) for in vitro embryo production (IVP) in crossbred heifers, two Latin square design studies investigated the effect of ovarian follicular wave synchronization with estradiol benzoate (EB) and progestins. For each experiment, crossbred heifers stage of estrous cycle was synchronized either with a norgestomet ear implant (Experiment 1) or a progesterone intravaginal device (Experiment 2) for 7 d, followed by the administration of 150 μg d-cloprostenol. On Day 7, all follicles >3 mm in diameter were aspirated and implants/devices were replaced by new ones. Afterwards, implant/device replacement was conducted every 14 d. Each experiment had three treatment groups. In Experiment 1 (n = 12), heifers in Group 2X had their follicles aspirated twice a week and those in Groups 1X and 1X-EB were submitted to OPU once a week for a period of 28 d. Heifers from Group 1X-EB also received 2 mg EB i.m. immediately after each OPU session. In Experiment 2 (n = 11), animals from Group 0EB did not receive EB while heifers in Groups 2EB and 5EB received 2 and 5 mg of EB respectively, immediately after OPU. The OPU sessions were performed once weekly for 28 d. Therefore, in both experiments, four OPU sessions were performed in heifers aspirated once a week and in Experiment 1, eight OPU sessions were done in heifers aspirated twice a week. Additionally, during the 7-d period following follicular aspiration, ovarian ultrasonography examinations were conducted to measure diameter of the largest follicle and blood samples were collected for FSH quantification by RIA. In Experiment 1, all viable oocytes recovered were in vitro matured and fertilized. Results indicated that while progestin and EB altered follicular wave patterns, this treatment did not prevent establishment of follicular dominance on the ovaries of heifers during OPU at 7-d intervals. Furthermore, the proposed stage of follicular wave synchronization strategies did not improve the number and quality of the recovered oocytes, or the number of in vitro produced embryos. |
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