Microsomal glucosidases acting on the saccharide moiety of the glucose-containing dolichyl diphosphate oligosaccharide |
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| Authors: | R A Ugalde R J Staneloni L F Leloir |
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| Affiliation: | 1. Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Universidad de Buenos Aires, Obligado 2490, Buenos Aires 1428, Argentina;2. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires 1428, Argentina |
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| Abstract: | The microsomal glucosidases which act on the oligosaccharide that contains 2 N-acetylglucosamine, 9 mannose and 1–3 glucose residues have been studied. Two fractions were separated by differential solubility in detergent and phosphate solutions or by gel filtration. One of the fractions removed glucose from the oligosaccharide containing three glucose residues, and another fraction acted on the compounds containing one and two glucose residues. Both fractions were free of mannosidase. Some properties of the enzymes are described. |
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