Reduction in the rate of DNA reassociation by sequence divergence |
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| Authors: | T I Bonner D J Brenner B R Neufeld R J Britten |
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| Affiliation: | 1. Carnegie Institution of Washington, Washington D.C. 20015 U.S.A;2. Walter Reed Army Institute of Research, Washington D.C. 20012 U.S.A;3. Division of Biology, California Institute of Technology, Pasadena, Calif. 91109, U.S.A. |
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| Abstract: | An estimate is made of the effect of imperfectly complementary sequences on the rate of reassociation of DNA. Rate measurements are reported for the reassociation of deaminated DNA and for the pairing of DNAs from related bacteria. A method is presented for separating the effect of the incubation temperature on the rate from the effect of sequence divergence. After correction to the optimum incubation temperature, the rate of DNA reassociation appears to be reduced by a factor of two for each 10 deg. C reduction in melting temperature due to sequence divergence. For most typical cases this effect is modest. However it can be quite important for measurements of the relation between the DNAs of different species. |
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