| 鲑鱼降钙素与骨生长肽融合基因的克隆及其在毕赤酵母中的表达 |
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| 引用本文： | 钟辉,彭英芳,李平,袁志刚,杨晓莉,马清钧. 鲑鱼降钙素与骨生长肽融合基因的克隆及其在毕赤酵母中的表达[J]. 中国生物工程杂志, 2002, 22(5): 79-82 |
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| 作者姓名： | 钟辉 彭英芳 李平 袁志刚 杨晓莉 马清钧 |
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| 作者单位： | 军事医学科学院生物工程研究所, 北京 100850; 武警总医院检验科, 北京 100039 |
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| 摘 要： | 降钙素 (Calcitonin ,CT)和骨生长肽 (Osteogenicgrowthpeptide ,OGP)是两种治疗骨质疏松症的药物 ,设想通过这两种基因的融合表达来达到治疗骨质疏松的目的。体外合成OGP和sCT的基因 ,经过DNA体外重组构建酵母表达质粒 ,实现了在毕赤酵母中的表达 ,通过纯化后进行氨基酸N端测序 ,证明表达的蛋白序列正确。
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| 关 键 词： | 骨生长肽 骨质疏松症 毕赤酵母 降钙素 |
The Cloning of Salmon Calcitonin and Osteogenic Growth Peptide Gene and Its Fusion Expression in Picha Yeasts |
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| Zhong Hui Peng Yingfang Li Ping Yuan Zhigang Yang Xiaoli Ma Qingjun. The Cloning of Salmon Calcitonin and Osteogenic Growth Peptide Gene and Its Fusion Expression in Picha Yeasts[J]. China Biotechnology, 2002, 22(5): 79-82 |
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| Authors: | Zhong Hui Peng Yingfang Li Ping Yuan Zhigang Yang Xiaoli Ma Qingjun |
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| Affiliation: | Zhong Hui Peng Yingfang Li Ping Yuan Zhigang Yang Xiaoli Ma Qingjun (Biotechnology Institute Beijing 100850)(People's republic army police general hospital Beijing 100850) |
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| Abstract: | To get fusion expression of Calcitonin (CT) and Growth Peptide (OGP) which have a clinical role in osteoporosis therapy.Six SCT and OGP DNA segments were chemically synthesized and ligated in a certain proportion into a yeast vector P PIC9 .The fusion gene of SCT+OGP was obtained,which was confirmed by sequence analysising.The recombinant plasmid was transformed into pichia pastoris GS115.Finally,we got two stable lineage of expressing protein.The primarily purified fusion protein was identified by amnio acid sequencing. |
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| Keywords: | Calcitonin OGP Osteoporosis Picha yeast |
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