Histological analysis of glucagon-like peptide-1 receptor expression in chicken pancreas |
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Authors: | Takafumi Watanabe Kei Nishimura Yoshinao Z Hosaka Takeshi Shimosato Shinichi Yonekura Daisuke Suzuki Chihiro Takemoto Mohammad Moniruzzaman Monir Kohzy Hiramatsu |
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Institution: | 1. Laboratory of Animal Functional Anatomy (LAFA), Department of Food Production Science, Faculty of Agriculture, Shinshu University, 8304 Minamiminowa-mura, Nagano, 399-4598, Japan 2. Department of Veterinary Anatomy, Tottori University, Tottori, 680-8553, Japan 3. Graduate School of Agriculture, Shinshu University, Nagano, 399-4598, Japan 4. Department of Animal Physiology, Faculty of Agriculture, Shinshu University, Nagano, 399-4598, Japan 5. Department of Musculoskeletal Biomechanics and Surgical Development, School of Medicine, Sapporo Medical University, Sapporo, Hokkaido, 060-0061, Japan
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Abstract: | Glucagon-like peptide-1 (GLP-1) released from intestinal L cells in response to nutrient ingestion inhibits both gastrointestinal emptying and gastric acid secretion and promotes satiety. The main biological effect of GLP-1 is the stimulation of insulin secretion (thereby fulfilling the criterion for an incretin hormone) in order to reduce blood glucose levels in mammalian species. Chicken GLP-1 receptor (cGLP-1R) has also been identified in various tissues by gene expression analysis. Although certain effects of GLP-1 in mammals and birds are consistent, e.g., inhibition of food intake, whether GLP-1 has the same insulinotropic activity in chickens as in mammals is debated. Moreover, the expression of cGLP-1R in chicken pancreatic B cells has not been reported. The localization of cGLP-1R and its mRNA in pancreatic islets is studied by triple-immunofluorescence microscopy and in situ hybridization. Triple-immunofluorescence microscopy with antisera against cGLP-1R, somatostatin and insulin or glucagon revealed that cGLP-1R protein was exclusively localized in D cells producing somatostatin in chicken pancreatic islets. The D cells were localized in peripheral areas of the pancreatic islets and cGLP-1R mRNA was detected in the same areas, indicating that cGLP-1R mRNA was also expressed in D cells. This is the first report to demonstrate that cGLP-1R is expressed by D cells, not B cells as in mammals. Our study suggests that chicken GLP-1 performs its insulinotropic activity by a different mode of action from that of the mammalian hormone. |
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