Up-Regulation of Podoplanin Involves in Neuronal Apoptosis in LPS-Induced Neuroinflammation |
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Authors: | Yan Song Jianhong Shen Yuchang Lin Jiabing Shen Xinming Wu Yaohua Yan Li Zhou Haiyan Zhang Ying Zhou Maohong Cao Yonghua Liu |
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Affiliation: | 1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China 2. Department of Neurology, Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China 3. Department of Neurosurgery, Affiliated Wuxi No. 2 Hospital of Nanjing Medical University, Wuxi, 214000, Jiangsu, People’s Republic of China 4. Department of Immunology, Medical College of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China 5. Department of Pathogen Biology Medical College, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China
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Abstract: | Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, information regarding its regulation and possible function in the central nervous system is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats and detected increased expression of PDPN in the brain cortex. Immunofluorescence indicated that PDPN was located in the neurons, but not astrocytes. Moreover, there was a concomitant up-regulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these three proteins in cortical primary neurons was decreased after knocking down PDPN by siRNA. Collectively, all these results suggested that the up-regulation of PDPN might be involved in neuronal apoptosis in neuroinflammation after LPS injection. |
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