|
|||||
|
|
Identification of PLP2 and RAB5C as novel TPD52 binding partners through yeast two-hybrid screening |
|
| Authors: | Hamideh Shahheydari Sarah Frost Brian J Smith Guy E Groblewski Yuyan Chen Jennifer A Byrne |
| Institution: | 1. Children’s Cancer Research Unit, Kids Research Institute, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, NSW, 2145, Australia 2. The University of Sydney Discipline of Paediatrics and Child Health, The Children’s Hospital at Westmead, Locked Bag 4001, Westmead, NSW, 2145, Australia 3. Department of Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia 4. Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA |
| Abstract: | Tumor protein D52 (TPD52) is overexpressed in different cancers, but its molecular functions are poorly defined. A large, low-stringency yeast two-hybrid screen using full-length TPD52 bait identified known partners (TPD52, TPD52L1, TPD52L2, MAL2) and four other preys that reproducibly bound TPD52 and TPD52L1 baits (PLP2, RAB5C, GOLGA5, YIF1A). PLP2 and RAB5 interactions with TPD52 were confirmed in pull down assays, with interaction domain mapping experiments indicating that both proteins interact with a novel binding region of TPD52. This study provides insights into TPD52 functions, and ways to maximise the efficiency of low-stringency yeast two-hybrid screens. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |