Extensive analysis of nuclear cistron rDNA sequence of <Emphasis Type="Italic">Paracyclopina nana</Emphasis> (Cyclopoida: Cyclopettidae) |
| |
| Authors: | Jang-Seu Ki Heum Gi Park Jae-Seong Lee |
| |
| Institution: | (1) Department of Green Life Science, College of Convergence, Sangmyung University, Seoul, 110-743, South Korea;(2) National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 133-791, South Korea;(3) Faculty of Marine Bioscience and Technology, College of Life Sciences, Kangnung National University, Gangneung, 210-702, South Korea; |
| |
| Abstract: | As a special reference of the nuclear cistron rDNA of cyclopoid copepods, we obtained the entire DNA sequence of a single
rDNA repeat unit from Paracyclopina nana (Cyclopoida: Cyclopettidae). The genomic organization of P. nana rDNA (7,974 bp, 51.7% of GC) was observed to be 18S–ITS1–5.8S–ITS2–28S–IGS in order. Comparative analyses of the rDNA between
P. nana and other copepods showed that the intergenic spacer (IGS) was highly informative and divergent, while other coding regions
are relatively conserved. We detected eleven poly(T) tracts in the IGS, demonstrating the high AT content in this region.
In addition, many sub-repeat sequence patterns (e.g., AG, AT, GC, CCG, TC) such as microsatellites were identified from the
rDNA IGS of P. nana. In this article, we show the first complete sequence of rDNA from the order Cyclopoida, providing a better understanding
of molecular characteristics in molecular taxonomy. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
