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Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration
Authors:Mohiuddin  AKM  Chowdhury  MKU  Abdullah  Zaliha C  Napis  Suhaimi
Institution:(1) Gentect, Faculty of Food Science and Biotechnology, University Pertanian Malaysia, 43400 Serdang, Selangor, Malaysia;(2) Porim, P.O. Box 10620, 50720 KL, Malaysia;(3) Present address: Department of Horticulture, University of Georgia, Athens, Georgia 30605, USA;(4) Craun, Lcda, Jln. Santubong, 93055 Kuching, Sarawak, Malaysia
Abstract:The effect of addition of silver nitrate (AgNO3) on organogenesis of proximal and distal cotyledon and hypocotyl explants of five cucumber (Cucumis sativus L.) cultivars was investigated. Distal cotyledon and hypocotyl were unresponsive while only poor shoot regeneration was observed in proximal cotyledon and hypocotyl explants of all cucumber cultivars. The addition of different concentrations of AgNO3 (10, 30 and 50 µM) to the medium, however, induced shoot regeneration in distal cotyledon except Suyo Long cultivar and effectively increased shoot regeneration response as well as the number of shoots per explant in proximal cotyledon and hypocotyl of all cucumber cultivars.
Keywords:cucumber  direct shoot regeneration  silver nitrate  tissue culture
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