Production of a novel raw-starch-digesting glucoamylase by <Emphasis Type="Italic">Penicillium</Emphasis> sp. X-1 under solid state fermentation and its use in direct hydrolysis of raw starch |
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Authors: | Haiyan Sun Xiangyang Ge Weiguo Zhang |
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Institution: | (1) The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 170# Huihe Road, Wuxi, 214036, JiangSu Province, P.R. China;(2) School of Biotechnology, Southern Yangtze University, 170# Huihe Road, Wuxi, 214036, JiangSu Province, P.R. China |
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Abstract: | Penicillium sp. X−1, isolated from decayed raw corn, produced high level of raw-starch-digesting glucoamylase (RSDG) under solid state
fermentation (SSF). Maximum enzyme yield of 306.2 U g−1 dry mouldy bran (DMB) was obtained after 36 h of culture upon optimized production. The enzyme could hydrolyse both small
and large granule starches but did not adsorb on raw starch. The enzyme exhibited maximum activity at 65°C and pH 6.5, which
provided an opportunity of synergism with α-amylase. It significantly hydrolysed 15% (w/v) raw corn starch slurry in synergism
with the commercial α-amylase and a degree of hydrolysis of 92.4% was obtained after 2 h of incubation. |
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Keywords: | Penicillium sp pH stable Raw-starch-digesting glucoamylase Solid state fermentation Synergism |
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