多花黄精诱导愈伤组织与不定芽培养基筛选

以多花黄精Polygonatum cyrtonema的根状茎为外植体，通过L9(34)正交试验比较不同植物生长调节剂及其组合对多花黄精愈伤组织诱导的影响，筛选最适生长调节剂配方，同时筛选通过器官发生方式直接成芽较为合适的培养基。结果表明，含有2,4-D和KT的培养基诱导愈伤组织效果显著，多花黄精愈伤组织诱导的最适培养基为MS + 6-BA 2.0 mg·L-1 + 2,4-D 0.5 mg·L-1 + NAA 0.1 mg·L-1 + KT 1.0 mg·L-1，该培养基的愈伤组织诱导率可达39.10%；培养基MS + 6-BA 4.0 mg·L-1 + NAA 0.2 mg·L-1可诱导多花黄精根状茎直接产生不定芽。

Screening Optimal Medium for Inducing Callus and Buds of Polygonatum cyrtonema

Rootstocks of Polygonatum cyrtonema were used as explants, and the effects of different plant growth regulators and their combinations on the callus induction were studied by L9(34) orthogonal test. The well medium was screened for directly buds formation by organogenesis of this medicinal plant. The results showed that the effect of callus induction was remarkable in medium containing 2,4-D and KT. The optimum medium for callus induction was MS + 6-BA 2.0 mg·L-1 + 2,4-D 0.5 mg·L-1 + NAA 0.1 mg·L-1 + KT 1.0 mg·L-1, and the callus induction rate reached up to 39.10%. The medium of MS + 6-BA 4.0 mg·L-1 + NAA 0.2 mg·L-1 was optimal for inducing adventitious buds.