The formation of an azo anion free radical metabolite during the microsomal azo reduction of sulfonazo III. |
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| Authors: | R P Mason F J Peterson J L Holtzman |
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| Affiliation: | Clinical Pharmacology Section, Veterans Administration Hospital, Minneapolis, Minnesota 55417; and Departments of Laboratory Medicine and Pathology, Medicinal Chemistry, Pharmacology, and Medicine, University of Minnesota, Minneapolis, Minnesota 55455 USA |
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| Abstract: | An ESR spectrum is observed during the anaerobic incubation of the diazonaphthol dye sulfonazo III, with rat hepatic microsomes and NADPH. This spectrum is characterized by a partially resolved 17-line hyperfine pattern and g = 2.0043, as is consistent with the spectrum of an azo anion free radical, [R-N-N-R′]. Oxygen, which strongly inhibits microsomal azoreductase, destroys the ESR signal. The oxidation of the azo anion radical metabolite by oxygen to the parent azo dye may account for the oxygen inhibition of microsomal azoreductase. |
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