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Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein. |
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| Authors: | P. R. Blake M. W. Day B. T. Hsu L. Joshua-Tor J. B. Park D. R. Hare M. W. Adams D. C. Rees M. F. Summers |
| Affiliation: | Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228. |
| Abstract: | The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability. |
| Keywords: | distance geometry hyperthermophile iron-sulfur protein NMR structure protein structure rubredoxin X-ray structure zinc substitution |