甘菊CMO同源基因的分离与表达分析

利用半嵌套巢式PCR结合RACE技术从菊科植物甘菊[Chrysanthemum lavandulifolium(Fisch.ex Trautv.)Makino]中分离得到了一个长度为1 450 bp的片段.序列分析结果表明,其开放阅读框全长1 140bp,编码379个氨基酸残基；在GenBank 比对并进行系统进化分析可知,该片段为CMO同源基因,命名为ClCMO.利用不同胁迫处理进行分析发现,在非胁迫条件下ClCMO基因在甘萄茎、叶、花叶中均有表达信号,在根中没有表达；其可以响应干旱、高盐胁迫和脱落酸(ABA)的诱导,不响应冷热胁迫,并且其表达在水杨酸(SA)诱导下受抑制.这些结果表明,ClCMO基因是提高植物耐干旱、高盐能力的有效基因资源.

Cloning and Expression Analysis of CMO Homologue Gene from Chrysanthemum lavandulifolium

Integrating heminested PCR with RACE(rapid-amplification of cDNA ends),a 1 140 bp segment was isolated from Chrysanthemum lavadulifolium.The sequence analysis showed that the gene consisted of an 1 140 bp ORF(open reading frame)which coded a protein with 379 amino acid residues.Based on Blast on GenBank and phylogenetic tree,the segment was found to be CMO homologue gene,and named as ClCMO.Under normal conditions,ClCMO expresses constitutively in leaves,stems,flowers,but not in root.After stress treatments,ClCMO can respond to drought,salt and ABA(abscisic acid)induction,and do not response to low or high temperature,in addition,it was inhibited by SA(salicylic acid).The results indicate that ClCMO can be regarded as one of an effective gene resource to improve the ability to resist drought and salinity.