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古尼拟青霉液体发酵液有机粗提物产量的研究
引用本文:李军进,郑永标,侯协锦,黄建忠.古尼拟青霉液体发酵液有机粗提物产量的研究[J].生物技术,2012,22(1):88-89.
作者姓名:李军进  郑永标  侯协锦  黄建忠
作者单位:工业微生物教育部工程研究中心,福建师范大学生命科学学院,福建省现代发酵技术工程研究中心,福建福州350108
基金项目:福建省科技计划重点项目,福建省教育厅科技项目
摘    要:目的:培养基成分对古尼拟青霉液体发酵液有机粗提物产量的影响。方法:采用正交设计方法,考察古尼拟青霉液体发酵培养基3种组分马铃薯、葡萄糖和蛋白胨对其菌丝体与发酵液有机粗提物量的影响。结果:在马铃薯300g/L,蛋白胨8g/L,葡萄糖30g/L时,古尼拟青霉液体发酵液有机粗提物产量可达到136.3mg/L。结论:培养基成分对古尼拟青霉液体发酵有机粗提物产量有显著影响,该文结果对指导古尼拟青霉液体的深层发酵具有参考价值。

关 键 词:古尼拟青霉  液体发酵  培养基  有机粗提物

Study on the Yield of Organic Crude from Paecilomyces gunnii by Liquid Submerged Fermentation
LI Jun-jin , ZHENG Yong-biao , HOU Xie-jin , HUANG Jian-zhong.Study on the Yield of Organic Crude from Paecilomyces gunnii by Liquid Submerged Fermentation[J].Biotechnology,2012,22(1):88-89.
Authors:LI Jun-jin  ZHENG Yong-biao  HOU Xie-jin  HUANG Jian-zhong
Institution:(Engineering Research Center of Industrial Microbiology Ministry of Education;College of Life Sciences; Engineering Research Center of Fujian Modern Fermentation Technology,Fujian Normal University,Fuzhou 350108,China)
Abstract:Objective:Effects of medium components on the yield of organic crude from Paecilomyces gunnii by liquid submerged fermentation.Method:Effects of three major medium compositions(potato,glucose and peptone) on the mycelia and organic crude amount from Paecilomyces gunnii by liquid submerged fermentation with orthogonal design were explored in this paper.Result:When the medium was the potato 300g/L,peptone 8g/L and glucose 30g/L,the yield of organic crude could be up to 136.3mg/L.Conclusion:The medium components play the important role in the yield of organic crude from Paecilomyces gunnii.This experiment layed the foundation for guiding liquid submerged fermentation of Paecilomyces gunnii.
Keywords:Paecilomyces gunnii  liquid submerged fermentation  medium  organic crude
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