一株河流污泥中产甲烷杆菌的生物学特性研究

目的:筛选产甲烷菌并对其进行鉴定和特性分析.方法:利用亨盖特厌氧操作技术从河流污泥中分离出的一株产甲烷杆菌.通过形态特征、生理生化、16S rRNA基因序列分析确定菌株的分类地位,利用气相色谱仪测定甲烷含量.结果:该菌株杆状,产芽孢,极端严格厌氧,宽约0.36μm,长约3.20μm,有时观察成链状,在液体培养的过程中,聚集成絮状沉积在管底.能够利用H2/CO2和甲钠盐生长,不利用甲醇、三甲胺、乙酸和二级醇类.最适生长温度范围为37℃,最适pH为7.5 ～8.0,能在0～1％盐浓度范围内生长.结论:菌株AG的最适温度较高,最适pH偏碱,在沼气发酵中具有重要应用前景.

Biological Characteristics of a Methanobacterium Isolated from River Sludge

Objective:To isolate and characterize Methanogen with growth characteristics Method:A strain AG was isolated from river sludge by hungate roll-tube technique.Through physiological,biochemical and phylogenetic analysis,the strain was identified.Metabolites were analyzed by gas chromatogram.Result:The strain is 0.36 × 3.20μm,rod,sometimes observed in chains,sometimes gathered into flocculent and deposited on the bottom of the tube in the course of liquid culture.Formate and H2 /CO2 could be utilized as substrate but not acetate,methanol,trimethylamine or secondary alcohols.Strain AG grew fastest at 37℃ and pH 7.5-8.0,and could grow with 0-1% NaCl.Conclusion:The optimum temperature of the strain AG is relatively high,while the strain can grow fastest within alkaline pH,which will posess significant potential for strain AG to be utilized in the methane fermentation.