| 缺陷短波单胞菌高产L-脯氨酸菌株的选育及其发酵条件优化 |
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| 引用本文： | 赵世杰,窦文芳,李会,张旦旦,史劲松,许正宏.缺陷短波单胞菌高产L-脯氨酸菌株的选育及其发酵条件优化[J].生物加工过程,2012,10(4):21-25. |
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| 作者姓名： | 赵世杰 窦文芳 李会 张旦旦 史劲松 许正宏 |
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| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,无锡214122;江南大学医药学院制药工程研究室,无锡214122
2. 江南大学医药学院制药工程研究室,无锡,214122 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目 |
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| 摘 要： | 以缺陷短波单胞菌(Brevundimonas diminuta)JNPP-1为出发菌株,利用紫外诱变及亚硝基胍复合诱变的方式,获得1株遗传性能稳定、可抗磺胺胍、能耐高渗透压以及能以琥珀酸钠为唯一C源的、在培养基上有生长优势的L-脯氨酸高产菌株JNPP-NSS(SGr、Sucg、NaClr)。经发酵条件优化后,L-脯氨酸的最高质量浓度可达47.3 g/L,与出发菌株相比提高了45.1%,生产速率由0.45 g/(L·h)提高到0.78 g/(L·h)。
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| 关 键 词： | L-缺陷短波单胞菌 发酵 L-脯氨酸 诱变选育 |
Breeding of Brevundimonas diminuta for L-proline and optimization of its fermentation conditions |
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| ZHAO Shijie
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DOU Wenfang
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LI Hui
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ZHANG Dandan
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SHI Jinsong
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XU Zhenghong.Breeding of Brevundimonas diminuta for L-proline and optimization of its fermentation conditions[J].Chinese Journal of Bioprocess Engineering,2012,10(4):21-25. |
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| Authors: | ZHAO Shijie DOU Wenfang LI Hui ZHANG Dandan SHI Jinsong XU Zhenghong |
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| Institution: | 1,2(1.Key Laboratory of Industrial Biotechnology of the Ministry of Education,Jiangnan University,Wuxi 214122,China; 2.Labaratory of Pharmaceutical Engineering,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Brevundimonas diminuta JNPP-1 was used as the original strain for stepwise mutation by ultraviolet(UV) and N-methyl-N-nitro-N-nitrosoguanidine(NTG) treatments.A stable and L-proline over-producing mutant JNPP-NSS(SGr、Sucg、NaClr) was obtained.Through the optimization of batch fermentation condition,the maximum concentration of L-proline reached 47.3 g/L,which was 45.1% higher than that of the original strain,and the production rate was increased from 0.45 to 0.78 g/(L·h). |
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| Keywords: | Brevundimonas diminuta fermentation L-proline mutagenesis |
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