| 木聚糖酶产生菌的筛选与酶学生化特性 |
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| 引用本文： | 李淑丽,王娇,尹清强,常娟,左瑞雨,王平,王潇.木聚糖酶产生菌的筛选与酶学生化特性[J].生物加工过程,2012,10(4):54-58. |
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| 作者姓名： | 李淑丽 王娇 尹清强 常娟 左瑞雨 王平 王潇 |
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| 作者单位： | 1. 河南农业大学 牧医工程学院,郑州,450002
2. 河南普爱饲料股份有限公司,周口,466000 |
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| 基金项目： | 河南省科技成果转化项目 |
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| 摘 要： | 从自然界中筛选出可分泌高产木聚糖酶的黑曲霉,并对其产酶条件和酶学特性进行优化和分析.结果表明:该菌株培养48 h后,所产的木聚糖酶比酶活最高,达到1645.66 U/g;通过培养基的优化,其比酶活达到了2 698.28 U/g.最优培养基组成(质量分数):麸皮75.0％、玉米12.5％、豆粕12.5％.该酶的最适温度为37℃,最适pH为4.5.
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| 关 键 词： | 黑曲霉 培养基 pH 温度 木聚糖酶 |
Isolation of xylanase-producing microbes and analysis of its biochemical properties |
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| LI Shuli
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WANG Jiao
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YIN Qingqiang
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CHANG Juan
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ZUO Ruiyu
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WANG Ping
,
WANG Xiao.Isolation of xylanase-producing microbes and analysis of its biochemical properties[J].Chinese Journal of Bioprocess Engineering,2012,10(4):54-58. |
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| Authors: | LI Shuli WANG Jiao YIN Qingqiang CHANG Juan ZUO Ruiyu WANG Ping WANG Xiao |
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| Institution: | 1.College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China; 2.Henan PUAI Feed Co.,Ltd.,Zhoukou 466000,China) |
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| Abstract: | The microbes with high levels of xylanase production were selected.The results showed that Aspergillus niger,xylanase was isolated with the high activity of 1 645.66 U/g at 48 h cultivatiion(P<0.05).Xylanase activity reached 2 698.28 U/g after medium selection(P<0.05).The optimal medium composition(in mass)was wheat bran 75.0%,corn 12.5%,and soybean meal 12.5%.The optimal temperature and pH were 37 ℃ and 4.5,respectively(P<0.05). |
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| Keywords: | Aspergillus niger media pH temperature xylanase |
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