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三种提取苹果绵蚜基因组DNA方法的比较
引用本文:武强,吕志创,万方浩,李照会. 三种提取苹果绵蚜基因组DNA方法的比较[J]. 生物技术通报, 2012, 0(1): 70-73,82
作者姓名:武强  吕志创  万方浩  李照会
作者单位:1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193
2. 山东农业大学植物保护学院,泰安,271018
摘    要:参考国内外昆虫基因组DNA提取的常用方法,选取KAc法、氯仿-异戊醇法和盐析法3种方法对苹果绵蚜(Eriosoma lanigerum)基因组DNA进行提取.通过基因组DNA直接琼脂糖凝胶电泳、SSR引物扩增产物的琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳检测,对3种方法所提基因组DNA的质量进行了比较,并综合分析了提取所需时间及所用试剂的毒性大小等.结果表明,盐析法操作程序较简便快捷,节时省工,可得较好质量的DNA样本,且对试验操作人员无伤害,是一种值得推广应用的基因组DNA提取方法.

关 键 词:昆虫  DNA提取  KAc法  氯仿-异戊醇法  盐析法  苹果绵蚜

Comparison of Three Methods for Genomic DNA Extraction from the Woolly Apple Aphid
Wu Qiang , Lü Zhichuang , Wan Fanghao , Li Zhaohui. Comparison of Three Methods for Genomic DNA Extraction from the Woolly Apple Aphid[J]. Biotechnology Bulletin, 2012, 0(1): 70-73,82
Authors:Wu Qiang    Lü Zhichuang    Wan Fanghao    Li Zhaohui
Affiliation:1State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193;2College of Plant Protection,Shandong Agricultural University,Taian 271018)
Abstract:Three methods to extract the genomic DNA of insects were implemented on the woolly apple aphid(Eriosoma lanigerum).All these methods can extract genomic DNA of good quality,which are suitable for the SSR-PCR amplification.Compared with the chloroform-isoamyl method and KAc method,the salting-out method spend less time,and is toxic free and easier to be carried out.Result showed that salting-out method is an easy,rapid,non-toxic and effective method to extract genomic DNA from insects.
Keywords:Insects DNA isolation KAc method Chloroform-isoamylol method Salting-out method Eriosoma lanigerum
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