| 甘蔗渣和糖蜜混合发酵制备燃料丁醇 |
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| 引用本文： | 杜腾飞,郭亭,汤艳,贺爱永,姜岷.甘蔗渣和糖蜜混合发酵制备燃料丁醇[J].生物加工过程,2012,10(4):12-15. |
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| 作者姓名： | 杜腾飞 郭亭 汤艳 贺爱永 姜岷 |
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| 作者单位： | 1. 南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京,210009
2. 南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009;广州甘蔗糖业研究所,广州510316 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，材料化学工程国家重点实验室基金，江苏省"青蓝工程"资助项目，林业公益性专项基金资助项目，国家自然科学基金青年基金资助项目 |
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| 摘 要： | 以抗逆突变株Clostridium beijerinckii IB4为研究对象,葡萄糖为C源,对其进行补料分批发酵过程的优化,同时将该优化工艺应用于甘蔗渣和糖蜜混合发酵制备燃料丁醇。结果表明:在5 L发酵罐中,先加入作为还原糖的甘蔗渣酸解糖液10 g/L,16 h后补加甘蔗糖蜜30 g/L,于35℃、100 r/min发酵50 h,丁醇和总溶剂产量分别达到11.1和15.3 g/L,丁醇比例高达72.5%。
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| 关 键 词： | 丁醇 Clostridium beijerinckii 补料分批 发酵 混合发酵 |
Production of butanol from bagasse and molasses by mixed fermentation |
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| DU Tengfei
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GUO Ting
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TANG Yan
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HE Aiyong
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JIANG Min.Production of butanol from bagasse and molasses by mixed fermentation[J].Chinese Journal of Bioprocess Engineering,2012,10(4):12-15. |
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| Authors: | DU Tengfei GUO Ting TANG Yan HE Aiyong JIANG Min |
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| Institution: | 1(1.State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China; 2.Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316,China) |
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| Abstract: | A tolerance mutant strain Clostridium beijerinckii IB4 was selected.Fed-batch fermentations were carried out in P2 fermentation medium with glucose,and the optimal fed-batch fermentation strategy was applied to butanol mixed fermentation from bagasse and molasses.Clostridium beijerinckii IB4 was cultured in a 5 L fermentor at 35 ℃,100 r/min for 50 h.The initial 10 g/L reducing sugar of acid hydrolysate of bagasse was added,and 30 g/L of molasses was fed into fermentation medium after 16 h.Butanol and total solvents concentrations accumulated in the broth were 11.1 and 15.3 g/L,respectively.Butanol proportion was up to 72.5%. |
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| Keywords: | butanol Clostridium beijerinckii fed-batch fermentation mixed fermentation |
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