| 纳米银复合介孔碳材料制备固定化猪胰脂肪酶 |
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| 引用本文： | 杨波,王伟,朱凯,王欢,韩萍芳.纳米银复合介孔碳材料制备固定化猪胰脂肪酶[J].生物加工过程,2012,10(3):50-55. |
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| 作者姓名： | 杨波 王伟 朱凯 王欢 韩萍芳 |
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| 作者单位： | 南京工业大学生物与制药工程学院,南京,210009 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目 |
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| 摘 要： | 以稻壳为原料制备介孔碳材料,对其表面进行纳米银修饰后作为猪胰脂肪酶(PPL)的固定化载体.初步研究并优化该载体固定猪胰脂肪酶的条件.结果表明:在AgNO3浓度为0.01 mol/L、固载时间6h、反应磷酸盐溶液pH为6.0、反应温度25℃时,可达最大酶活,酶活提高3.2倍.同时利用红外图谱(FT-IR)、N2等温吸附-脱附曲线(BET)和扫描电子显微镜(SEM)的分析手段对固定化猪胰脂肪酶试样进行分析,进一步确定了纳米银复合介孔性碳材料在固定化酶实验中的作用.
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| 关 键 词： | 介孔碳材料 固定化 纳米银 猪胰脂肪酶 |
Porcine pancreatic lipase immobilised on nano-silver mesoporous activated carbon composite |
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| YANG Bo
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WANG Wei
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ZHU Kai
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WANG Huan
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HAN Pingfang.Porcine pancreatic lipase immobilised on nano-silver mesoporous activated carbon composite[J].Chinese Journal of Bioprocess Engineering,2012,10(3):50-55. |
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| Authors: | YANG Bo WANG Wei ZHU Kai WANG Huan HAN Pingfang |
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| Institution: | (College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | The study was carried out to immobilize porcine pancreatic lipase(PPL)on nano-silver mesoporous activated carbon(Ag-MAC).MAC was prepared from rice husk by a two stage process and nano-silver was absorbed on the surface.PPL was then immobilized on Ag-MAC and conditions were optimized.Results showed that under the conditions of the silver nitrate concentration of 0.01 mol/L,immobilization time of 6 h,reactive phosphate solution of pH 6.0,reaction temperature 25 ℃,the lipase activity increased by 3.2 times.Meanwhile,N2 adsorption-desorption(BET),scanning electron microscopy(SEM),Fourier transform-infrared spectroscopy(FT-IR) were used to analyze experimental results,and confirm the effects of Ag-MAC in experiments of lipase immobilization. |
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| Keywords: | mesoporous activated carbon immobilization nano-silver porcine pancreatic lipase |
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