| 一株阿尔泰虫草内生菌的分离及抑菌活性 |
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| 引用本文： | 马赟,索菲娅,王傲立,黄罗冬,叶星,张琳,李萍,龚健. 一株阿尔泰虫草内生菌的分离及抑菌活性[J]. 生物技术, 2012, 22(2): 64-67 |
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| 作者姓名： | 马赟 索菲娅 王傲立 黄罗冬 叶星 张琳 李萍 龚健 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆乌鲁木齐,830046 |
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| 基金项目： | 新疆教育厅项目，新疆科技厅项目，自治区绿洲生态重点实验室资助项目 |
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| 摘 要： | 目的:从采自阿尔泰山脉的虫[ Ophiocordyceps gracilis( Grev.)G.H Sung,J.M.Sung,Hywel- Jones&Spatafora]体内分离出1株出现频率较高的真菌(命名为SFY00C3)进行了研究.方法:经形态学和ITS序列分析鉴定,对SFY00C3菌株及其发酵液采用滤纸片法,同时对6种供试菌株不同程度进行了抑制作用的研究.结果:SFY00C3菌株为青霉属(Penicillium)真菌变灰青霉(Penicillium canescens)；对大肠杆菌和金黄色葡萄球菌的抑制作用较强,抑菌圈均大于10 mm.结论:虫草内生菌变灰青霉的抑菌活性成分值得进一步研究,为研究新的天然产物抑菌药物提供思路.
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| 关 键 词： | 阿尔泰虫草 内生菌 分离 抑菌活性 |
Segregation and Bacteriostasis Research of Endophytes from Ophiocordyceps gracilis ( Grev.) G.H.Sung ,J.M.Sung ,Hywel -Jones & Spatafora |
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| MA Yun , SUO Fei-ya , WANG Ao-li , HUANG Luo-dong , YE Xing , ZHANG Lin , LI Ping , GONG Jian. Segregation and Bacteriostasis Research of Endophytes from Ophiocordyceps gracilis ( Grev.) G.H.Sung ,J.M.Sung ,Hywel -Jones & Spatafora[J]. Biotechnology, 2012, 22(2): 64-67 |
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| Authors: | MA Yun SUO Fei-ya WANG Ao-li HUANG Luo-dong YE Xing ZHANG Lin LI Ping GONG Jian |
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| Affiliation: | (College of Life Science and Technologe,Xinjiang University,Urumqi 830046,China) |
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| Abstract: | Objective:A strain of fungal seperated form Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora was highly frequently appeared,and was researched in this experiment.Method:To classify this fungal,morphology evidence and ITS sequencing was applied.Various bacteriostasis ability of this fungal and its broth against 6 strains was tested by the filter paper method too.Result:SFY00C3 is Penicillium canescen,whose broth performed a stronger bacteriostasis to Escherichia coli and Staphylococcus aureus as creating inhibition zone exceed 10mm in diameter.Conclusion:Penicillium canescens,the entophyte of Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora,is worthwhile further researching to offer new means of developing new natural anti-bacterial drugs. |
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| Keywords: | Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora entophyte isolate bacteriostasis activity |
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