环介导等温扩增技术的研究进展

环介导等温扩增(loop-mediated isothermal amplification,LAMP)是一种新式核酸扩增技术,它依靠一种具有链置换活性的DNA聚合酶和2对特殊设计的引物,不需要反复的温度循环和昂贵的仪器设备,在等温条件下即可高效快速地完成扩增反应,目前已广泛应用于细菌、病毒、寄生虫等病原体的检测,及动物胚胎性别的鉴定。该文总结了LAMP技术的基本原理、相对于传统核酸检测技术的优点、产物的检测方法及其临床应用,最后指出LAMP目前存在的不足以及采取的相应措施,并对其发展前景进行了展望。

Advances of Loop - Mediated Isothermal Amplification

Loop-mediated isothermal amplification(LAMP) which relys on a DNA polymerase with strand displacement activity and two pairs of specially designed primers is an novel nucleic acid amplification method,no repeated temperature cycling,no expensive equipment,the LAMP reaction was carried out under isothermal conditions with a high efficiency and a high speed,it has been widely applied to the detection of such pathogens as bacteria,virus,parasite etc,as well as sexing of animal embryos.The basic principle,advantages relatived to other gene amplification techniques,the methods to judge target sequence as well as application of LAMP were reviewed,finally,some existing problems and corresponding measures and prospects for LAMP were discussed in order to provide reference for reader studying.