S-腺苷蛋氨酸和谷胱甘肽联产发酵中盐胁迫的作用

摇瓶条件下考察不同的盐(NaCl,Na2SO4,KCl,K2SO4)胁迫对产朊假丝酵母发酵联产S-腺苷蛋氨酸(SAM)和谷胱甘肽(GSH)的影响。结果发现适当浓度的Na+和K+对SAM和GSH合成具有部分促进作用,而Cl-的作用则相反。以Na2SO4为代表,考察分批发酵条件下盐胁迫的作用,结果表明:在酵母细胞生长后期(15 h)添加10 g/L Na2SO4,SAM、GSH以及二者的最大联产量为252.5、285.9和521.9 mg/L,比对照分别提高了8.8%、22.6%和13.9%。最后分别从能量代谢和发酵动力学角度对分批发酵的结果进行了分析。

Co-production of S-adenosylmethionine and glutathione under salt-stress by Candida utilis

Effects of salt stress of NaCl,Na2SO4,KCl and K2SO4 on co-production of S-adenosylmethionine(SAM) and glutathione(GSH) was investigated in flaske by Candida utilis CCTCC M 209298.It was shown that Na+and K+ enhanced the co-production of SAM and GSH on appropriate concentrations,while Cl-inhibited cell growth and product biosynthesis.Based on the results,salt stress resulted from Na2SO4 was further studied under batch cultivation.The concentrations of SAM,GSH,the co-production of SAM,and GSH reached 252.5 mg/L,285.9 mg/L and 521.9 mg/L after adding 10 g/L of Na2SO4 at 15 h were increased by 8.8%,22.6% and 13.9%,respectively.Compared with those without Na2SO4 addition.Furthermore,the results were analyzed by concentrating on the energy metabolism and the fermentative kinetics in batch fermentation of SAM and GSH.