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| 低聚木糖的提取工艺及相对分子质量分布 | |
| 引用本文： | 隋明,荣加超,张阳,曲鹏,祝玉松,张丽萍.低聚木糖的提取工艺及相对分子质量分布[J].生物加工过程,2012,10(3):45-49. |
| 作者姓名： | 隋明 荣加超 张阳 曲鹏 祝玉松 张丽萍 |
| 作者单位： | 1. 黑龙江八一农垦大学食品学院,大庆,163319 2. 黑龙江八一农垦大学经济管理学院,大庆,163319 3. 东北石油大学电气信息工程学院,大庆,163319 |
| 基金项目： | 黑龙江省科技厅攻关计划资助项目 |
| 摘 要： | 通过单因素试验和正交试验研究低聚木糖的提取条件,使用凝胶树脂对低聚木糖粗提液进行分离,采用高效液相色谱法测定了低聚木糖的相对分子质量分布。低聚木糖的最佳提取条件:在底物质量浓度为100 g/L的情况下,加酶量1 000 U/g,酶解温度55℃,提取时间为4 h。在此条件下,提取的平均聚合度为3.12,高效液相色谱测定结果发现,低聚木糖主要是由木二糖、木三糖以及4～8个聚合度的糖组成。 |
| 关 键 词： | 低聚木糖 分子量分布 酶法水解 |
Extraction and molecular weight distribution of xylooligosaccharides |
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| SUI Ming , RONG Jiachao , ZHANG Yang , QU Peng , ZHU Yusong , ZHANG Liping.Extraction and molecular weight distribution of xylooligosaccharides[J].Chinese Journal of Bioprocess Engineering,2012,10(3):45-49. | |
| Authors: | SUI Ming RONG Jiachao ZHANG Yang QU Peng ZHU Yusong ZHANG Liping |
| Institution: | 1(1.College of Food Science,Heilongjiang Bayi Agriculture University,Daqing 163319,China; 2.College of Economics and Management,Heilongjiang Bayi Agriculture University,Daqing 163319,China; 3.College of Electronical Engineering and Information,Northeast Petroleum University University,Daqing 163319,China) |
| Abstract: | The optimization of extraction technique of xylooligosaccharides was studied with the monofactorial experienment and orthogonal test.After purified with gelatin resin,the molecular weight distributions of xylooliosaccharides were determined by HPLC.The result showed that the best technological parameters: the concentration of substrate on xylooligosaccharide contents was 100 g/L,the amount of the xylanase was 1 000 U/g,enzyme reaction temperature were at 55 ℃,extraction duration was 4 h.The determination results showed that xylobiose,xylotriose and the 4-8 polymerization degree of sugar were main ingredients in xylooligosaccharides. |
| Keywords: | xylooligosaccharide molecular weight distribution enzymatic hydrolysis |
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