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RAPD-PCR analysis of genetic diversity in the Manchurian pheasant
Authors:Kulikova I V  Chelomina G N  Zhuravlev Iu N
Affiliation:Institute of Biology and Soil Science, Russian Academy of Sciences, Vladivostok, 690022 Russia.
Abstract:The genetic diversity of a local population of the Manchurian pheasant Phasianus colchicus pallasi was studied using RAPD-PCR. Based on the DNA patterns obtained in PCR with five arbitrary decanucleotide primers, we assessed genetic polymorphism of this population, estimated genetic distances between individuals, and constructed an NJ phylogenetic tree, and an UPGMA dendrogram of genetic similarity. The population was shown to exhibit high average genetic polymorphism (P = 79.4%) and genetic distances (D = 0.267). Possible reasons for the high genetic diversity of this local population are discussed.
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