Microsatellite repeat dynamics in mitochondrial genomes of phytopathogenic fungi: frequency and distribution in the genic and intergenic regions |
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| Authors: | Sahil Mahfooz Pallavi Singh Deepak K Maurya Mahesh C Yadav Azram Tahoor Harmesh Sahay Arpita Srivastava Anil Prakash |
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| Institution: | 1Department of Biotechnology and Bioinformatics, Barkatullah University, Bhopal 462 026, Madhya Pradesh, India;2National Bureau of Agriculturally Important Microorganisms, Mau 275 101, Uttar Pradesh, India;3National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India;4Department of Wildlife Science, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India |
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| Abstract: | The frequency and distribution of microsatellites were analyzed in the 19 mitogenomes of phytopathogenic fungi covering five
phyla. Our analysis revealed that in all the mitogenomes studied, the frequency and relative abundance varied, and it was neither
influenced by genome size nor by GC content. SSRs were found to be differential distributed in genic and intergenic regions. An
average of 5.14 (23.6%) SSRs were present in genic sequences and 21.7 (76.4%) SSRs were located in the intergenic sequences.
Relative abundance of SSRs in mitogenomes was the highest in Aspergillus tubigensis, whereas, it was the least in Phaeosphaeria
nodurum, the average being 0.45. Trinucleotide repeats were the most abundant motifs in the genic and intergenic regions of the
mitogenomes of the phytopathogenic fungi. Among the genes, cox1 harbors the maximum SSRs, whereas cox3 and nad 7 contain the
least. Based on the presence of SSRs in a particular gene, genetic relationships among individual organisms were also established. |
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| Keywords: | Simple sequence repeats Comparative genomics Mitochondria Phytopathogenic fungi Phylogenetics |
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