The Sec-1 locus on the short arm of chromosome 1R of rye (Secale cereale) |
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| Authors: | B C Clarke Y Mukai R Appels |
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| Institution: | (1) Division of Plant Industry, C.S.I.R.O., P.O. Box 1600, 2601, A.C.T., Australia;(2) Laboratory of Plant Molecular Genetics, Osaka Kyoiku University, 4-698-1 Asahigaoka, 582 Kashiwara Osaka, Japan |
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| Abstract: | This paper describes a detailed sequence analysis of the ω-secalin gene array at theSec-1 locus on the short arm of chromosome 1 of rye. The analysis shows that the genes are separated by 8 kb of spacer sequence
and that the gene/spacer units are arranged in a head to tail fashion. The boundaries of the array are identified, and a fragment
containing the majority of the genes in the array is separated by PFG analysis. The sequence data of one 9.2 kb gene unit
have been determined, and because of the similarity of the gene units within the array these data provide a detailed sequence
analysis of 140 kb of theSec-1 locus. Fluorescence in situ hybridization, using lambda clones isolated for the structural analysis, identifies the position
of the array on the rye chromosomes relative to the 5S rRNA genes.
Edited by: W. Hennig |
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