Molecular cloning and characterization of the complementary DNA coding for the B-chain of murine Clq |
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| Authors: | F Petry K B Reid M Loos |
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| Affiliation: | Institute of Medical Microbiology, Johannes Gutenberg-University, Mainz, FRG. |
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| Abstract: | cDNA clones coding for the B-chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B-chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate-activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues. |
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