Synthesis of a convenient thymidine glycol phosphoramidite monomer and its site-specific incorporation into DNA fragments |
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| Authors: | Gasparutto Didier Cognet Sonia Roussel Solveig Cadet Jean |
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| Affiliation: | Labratoire des Lésions des Acides Nucléiques, Service Chimie Inorganique Biologique, UMR-E3 CEA-UJF, DRFMC, CEA Grenoble, France. dgasparutto@cea.fr |
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| Abstract: | An original phosphoramidite building block of the thymidine glycol lesion has been prepared taking into account the additional diol function and the high lability of this oxidatively induced nucleobase damage. Then the modified nucleoside was site-specifically inserted into DNA fragments by solid support assembling followed by a "one-step" mild final deprotection treatment. |
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