Protein encapsulation in liposomes: efficiency depends on interactions between protein and phospholipid bilayer.期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Protein encapsulation in liposomes: efficiency depends on interactions between protein and phospholipid bilayer.

Authors:	Jacques-Philippe?Colletier,Barnabé?Chaize,Mathias?Winterhalter,Didier?Fournier author-information" > author-information__contact u-icon-before" > mailto:fournier@cict.fr" title=" fournier@cict.fr" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	1.Laboratoire de Synthèse et Physicochimie des Molécules d'Intérêt Biologiques – Groupe de Biochimie des Protéines,Université Paul Sabatier,Toulouse,France;2.Institut de Pharmacologie et de Biologie Structurale – Laboratoire de Biophysique Membranaire,Toulouse,France

Abstract:	Background We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, however, are fragile and encapsulation requires in addition the preservation of their functionality. Using acetylcholinesterase as a model, we found that most protocols lead to a rapid denaturation of the enzyme with loss in the functionality and therefore inappropriate for such an application. The most appropriate method is based on lipid film hydration but had a very low efficiency.

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