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Recycling and adaptation of Klebsormidium flaccidum microalgae for the sustained production of gold nanoparticles |
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| Authors: | Dahoumane Si Amar Djediat Chakib Yéprémian Claude Couté Alain Fiévet Fernand Coradin Thibaud Brayner Roberta |
| Affiliation: | 1. Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, 15 rue Jean de Ba?f, F‐75205 Paris Cedex 13, France;2. telephone: 33‐11‐57‐27‐87‐64;3. fax: 33‐11‐57‐27‐72‐63;4. Muséum National d'Histoire Naturelle, Département RDDM, FRE 3206, USM 505, 57 rue Cuvier, F‐75005 Paris, France;5. UPMC Univ Paris 06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcellin Berthelot, F‐75005 Paris, France |
| Abstract: | Targeting the development of cell-based bioreactors for the production of metal nanoparticles, the possibility to perform the sustained synthesis of colloidal gold using Klebsormidium flaccidum green algae was studied. A first strategy relying on successive growth/reduction/reseeding recycling steps demonstrated maintained biosynthesis capability of the microalgae but limitation in metal content due to toxic effects. An alternative approach consisting of progressive gold salt addition revealed to be suitable to favor cell adaptation to larger metal concentrations and supported particle release over month periods. |
| Keywords: | gold nanoparticles photosyntetic activity green microalgae living materials |
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