东湖浮游生物群落DNA指纹结构及其与环境理化因子的关系

对武汉东湖5个不同湖区的浮游生物群落DNA进行了RAPD指纹分析，并探讨了DNA指纹结构与环境理化因子的关系．结果表明：所筛选的9条随机引物共扩增210条大小为150～2000bp的谱带，多态率为93．3％．各站点平均有42条谱带，其中Ⅳ站最多（53条），Ⅴ站最少（35条）．Ⅰ站的PO4^3--P、TP含量最高，Ⅴ站的NH4^＋-N、TN、NO2^--N含量最高，Ⅳ站各理化因子含量均低于其他站点，站点间COD、碱度、硬度、钙含量差异不大．相似性聚类分析表明，基于RAPD标记的浮游生物群落指纹将5个站点划分为两类：Ⅰ、Ⅱ、Ⅲ站聚为一枝，Ⅳ、Ⅴ站聚为另一枝．这与湖区主要理化因子的聚类结果一致．说明东湖不同湖区浮游生物群落DNA指纹与其环境理化因子密切相关．

DNA fingerprinting structure of plankton community and its relations to environmental physical-chemical factors in Donghu Lake

By the method of RAPD fingerprinting, this paper studied the DNA fingerprinting structure of plankton community and its relations to the main environmental physical-chemical factors at five sites in Donghu Lake. From the screened 9 random primers, a total of 210 observable bands with a length of 150-2 000 bp were amplified, 93.3% of which were polymorphic. At the five sites, the average number of amplified bands was 42, with the maximum (53) at site IV and the minimum (35) at site V. The PO4(3-)-P and TP contents were the highest at site I, NH4(+)-N, TN and NO2(-)-N contents were the highest at site V, while the values of all test physical-chemical parameters were the lowest at site IV. No obvious differences in COD, alkalinity, rigidity, and calcium content were observed among the study sites. Similarity clustering analysis showed that the DNA fingerprinting of plankton community based on RAPD marker could cluster the five sites into two groups, i. e., sites I, II and III could be clustered into one group, while sites IV and V could be clustered into another group, which was consistent with the clustering analysis based on the main environmental physical-chemical factors. In conclusion, there was a close relation between the DNA fingerprinting structure of plankton community and the main environmental physical-chemical factors in Donghu Lake.