甲状腺素对小鼠IgM的产生及淋巴细胞上β—肾上腺素能受体的影响

本文利用实验性“甲高”和“甲低”小鼠模型,观察了甲状腺素(L-T4)对绵羊红细胞(SRBC)诱导小鼠脾细胞产生 IgM 的影响。并采用放射配体结合法,测定了不同甲状腺机能状态的小鼠在免疫反应过程中,脾淋巴细胞上 β-肾上腺素能受体(β-Adr.R.)的密度及亲和力的变化。结果发现,用 SRBC 免疫后第3—5天,甲高小鼠脾细胞产生的 IgM 及脾淋巴细胞上β-Adr.R.密度的增加均明显高于对照小鼠(P<0.01),甲低小鼠则相反,其 IgM 的生成及脾淋巴细胞上β-Adr.R.密度的增加均明显低于对照小鼠(P<0.01),受体亲和力不随小鼠甲状腺机能状态及免疫状态的改变而改变(P>0.05)。以上结果提示,L-T_4能促进小鼠对SRBC 的免疫反应,其机制可能与淋巴细胞上 β-Adr.R.的密度变化有关。

Effects of thyroxine on IgM production and beta-adrenergic receptor on lymphocyte in mice

The effects of thyroxine (L-T4) on the IgM production of splenocytes in mice induced by sheep red blood cell (SRBC) were investigated by means of spectrophotometry of quantitative hemolysis (QHS). The maximum binding site number (Bmax) and affinity (Kd) of beta-adrenergic receptors (beta-Adr. R.) on splenic lymphocytes of mice under the different thyroid functional status during immune response were determined by radio-ligand combination method. The IgM production and Bmax of beta- Adr. R. of splenic lymphocytes were increased in "hyperthyroid mice" and decreased in "hypothyroid mice" as compared with the control (P less than 0.01) during the 3rd to 5th day after immunization with SRBC, but affinity of beta-Adr. R. was not changed (P greater than 0.05). It is suggested that L-T4 could increase the IgM production in lymphocytes induced by SRBC in mice. The underlying mechanism seems to involve an increasing Bmax of beta-Adr. R. on the immune cell, thus enhancing its sensitivity to catecholamine.