Injury-Induced Enzymatic Methylation of Aging Collagen in the Extracellular Matrix of Blood Vessels |
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Authors: | Darin J. Weber and Philip N. McFadden |
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Affiliation: | (1) Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA |
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Abstract: | As a result of blood vessel injury, protein D-aspartyl/L-isoaspartyl carboxyl methyltransferase (PIMT), a normally intracellular enzyme, becomes trapped within the meshwork of the vascular extracellular matrix where it can methylate substrate proteins. In this investigation we examined the distribution of such altered aspartyl-containing substrate proteins in the vascular wall. Nearly 90% of all the altered aspartyl residues were inaccessible to intracellular PIMT. Proteins of the extracellular matrix were found to be the major repository of altered aspartyl-containing polypeptides in the blood vessel wall, accounting for 70% of the total amount. Proteolytic cleavage of extracellular matrix proteins with cyanogen bromide (CNBr) revealed that collagens account for most of the altered aspartyl-containing proteins of the ECM. As a consequence of blood vessel injury, both type I and type III collagen along with other proteins were found to become methylated by injury-released PIMT. It is estimated that 1 cm of vein contains on the order of 5×1014 altered aspartyl residues involving between 1% and 5% of the total extracellular protein. |
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Keywords: | Protein (D-aspartyl/L-aspartyl) carboxy methyltransferase aging isoaspartyl HPLC electrophoresis AdoMet |
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