| Affiliation: | (1) National Institute for Plant Biodiversity Conservation and Research, Nayapalli, 751015 Bhubaneswar, Orissa, India;(2) Regional Plant Resource Centre, Nayapalli, 751015 Bhubaneswar, Orissa, India;(3) Centre for Environmental Management of Degraded Ecosystems, School of Environmental Studies, North Campus, University of Delhi, 110 007 Delhi, India;(4) Electron Microscopy Facility, All India Institute of Medical Sciences, 110019 New Delhi, India |
| Abstract: | A significant decrease in the amount of a protein, whose migration in two-dimensional gel electrophoresis corresponds to an apparent molecular mass of 23 kDa and pI=6.5, was observed in leaves of NaCl-treated Bruguiera parviflora (Roxb.) Wt. & Arn. ex Griff. seedlings. This particular salt-sensitive protein, designated as SSP-23, almost disappeared after 45 days of treatment in 400 mM NaCl as compared to untreated seedlings (0 mM NaCl) where the presence of the protein was significant. A polyclonal antibody raised against the 23-kDa protein was used to determine the subcellular localization of this protein in leaves by cross-reaction with proteins from isolated chloroplasts, mitochondria, peroxisomes and cytosol fractions on Western blots. SSP-23 was confirmed to be localized in the cytosol by immunoblotting. The disappearance of SSP-23 as a result of high NaCl treatment suggests that this protein is salt-sensitive and has a possible role in salt adaptation. |
