超临界CO_2萃取韭菜籽油成分的GC-MS分析

以索氏提取法为对照,采用超临界二氧化碳(SC-CO_2)萃取韭菜籽油,气相色谱-质谱联用技术(GC-MS)对韭菜籽油成分进行分析,NIST 02质谱数据库对其进行分析和鉴定.结果表明,SC-CO_2萃取压力为22.25 MPa、温度为40.40℃条件下萃取86.7 min时,萃取得率为17.52%,共分离鉴定出17种物质,其中,饱和脂肪酸以棕榈酸(6.25%)为主,占脂肪酸总量的 9.05%;不饱和脂肪酸主要是亚油酸(69.71%)和油酸(19.53%),占脂肪酸总量的90.50%.采用索氏提取得率为16.50%,共鉴定出10种物质,饱和脂肪酸以棕榈酸(7.22%)为主,占总脂肪酸量的9.84%;不饱和脂肪酸主要是亚油酸(69.34%)和油酸(20.12%),不饱和脂肪酸占脂肪酸总量的90.16%.另外SC-CO_2萃取韭菜籽油还检出单不饱和脂肪酸7-棕榈烯酸、角鲨烯和β-谷甾醇.

Seed Oil Supercritical Carbon Dioxide Extraction of Allium tuberosum Rottl.and Identification of Oil Compounds with GC-MS

Supercritical carbon dioxide (SC-CO_2) extract Allium tuberosum Rottl.seeds oil,Soxhlet method extraction as control experiment,gas chromatography-mass spectrometry (GC-MS) were employed to assay the oil chemical components in A.tuberosum Rottl.seed and NIST 02 spectral database were used to analyze and identify these components.SC-CO_2 extraction parameters were:pressure (22.25 MPa),temperature (40.40℃) and time (86.70 min),the value of oil extraction from A.tuberosum Rottl.seeds was 17.52%.17 compositions of seeds oil were identified by GC-MS.The totally fatty acids in the extracts compose of 9.05% saturated fatty acids and 90.50% unsaturated fatty acids.Furthermore,linoleic (69.71%),oleic (19.53%) and palmitic (6.25%) were found the most abundant fatty acids in SC-CO_2 extraction seeds oil.The value of Soxhlet method extraction oil from A.tuberosum Rottl.seeds was 16.50%.10 compositions of seeds oil were identified by GC-MS.Lnoleic (69.34%),oleic (20.12%) and palmitic (7.22%) were found the most abundant fatty acids in seed oil.The totally fatty acids in the Soxhlet method extracts composed of 9.84% saturated fatty acids and 90.16% unsaturated fatty acids.In addition,the monounsaturated fatty acid (7-Hexadecenoic acid),squalene and β-sitosterol were found in SC-CO_2 extraction oil of A.tuberosum Rottl.seeds.