坛紫菜原生质体的发育研究

用2％的海螺酶和1％的纤维素酶混合,将坛紫菜叶状体的4种不同细胞类型即:营养细胞、根丝细胞、精子囊和果孢子囊,分别解离成原生质体,并研究了这些不同部位和不同生长时期。细胞的生长、发育途径。由根丝细胞分离的原生质体能长成新的叶状体;由早期中部营养细胞分离的原生质体,有70％长成新的叶状体,其余的发育成精子囊和果孢子囊。再生叶状体在室内培养,能正常成熟。由精子囊和果孢子囊分离的原生质体,即精子细胞和果孢子细胞不能再生叶状体。前者形成新的精子囊放散精子,后者形成新的果孢子囊,放散果孢子发育成丝状体。晚期紫菜与早期紫菜比较,再生叶状体的数量显著减少,而发育成精子囊和果孢子囊的数量则大大增多。

Developmental Studies on Protoplasts of Porphyra haitanenns

Different types of protoplasts were isolated respectively from the thallus of P.haitanensis by enzymatic method.Most of the protoplasts from vegetative area could regenerate thalli,but a small part of them formed genital cells (carpogonia or spermatia).Protoplasts from sper-matangia and carposporangia could not regenerate thalli,the former developed spermatia,the latter developed carpospores.In comparison with younger thalli,the number of regenerated thalli decreased greatly,while the number of genital cells increased when old parent thalli were used.