Mapping the whole human genome by fingerprinting yeast artificial chromosomes. |
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Authors: | C Bellanné-Chantelot B Lacroix P Ougen A Billault S Beaufils S Bertrand I Georges F Glibert I Gros G Lucotte |
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Affiliation: | Centre d'Etude du Polymorphisme Humain, Paris, France. |
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Abstract: | Physical mapping of the human genome has until now been envisioned through single chromosome strategies. We demonstrate that by using large insert yeast artificial chromosomes (YACs) a whole genome approach becomes feasible. YACs (22,000) of 810 kb mean size (5 genome equivalents) have been fingerprinted to obtain individual patterns of restriction fragments detected by a LINE-1 (L1) probe. More than 1000 contigs were assembled. Ten randomly chosen contigs were validated by metaphase chromosome fluorescence in situ hybridization, as well as by analyzing the inter-Alu PCR patterns of their constituent YACs. We estimate that 15% to 20% of the human genome, mainly the L1-rich regions, is already covered with contigs larger than 3 Mb. |
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