Computational structural and functional analysis of hypothetical proteins of Staphylococcus aureus

Genome sequencing projects has led to an explosion of large amount of gene products in which many are of hypothetical proteinswith unknown function. Analyzing and annotating the functions of hypothetical proteins is important in Staphylococcus aureuswhich is a pathogenic bacterium that cause multiple types of diseases by infecting various sites in humans and animals. In thisstudy, ten hypothetical proteins of Staphylococcus aureus were retrieved from NCBI and analyzed for their structural and functionalcharacteristics by using various bioinformatics tools and databases. The analysis revealed that some of them possessed functionallyimportant domains and families and protein-protein interacting partners which were ABC transporter ATP-binding protein,Multiple Antibiotic Resistance (MAR) family, export proteins, Helix-Turn-helix domains, arsenate reductase, elongation factor,ribosomal proteins, Cysteine protease precursor, Type-I restriction endonuclease enzyme and plasmid recombination enzymewhich might have the same functions in hypothetical proteins. The structural prediction of those proteins and binding sitesprediction have been done which would be useful in docking studies for aiding in the drug discovery.