Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry |
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| Authors: | Cory Bystrom Shijun Sheng Ke Zhang Michael Caulfield Nigel J. Clarke Richard Reitz |
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| Affiliation: | 1Cleveland Heart Lab, Cleveland, Ohio, United States of America;2Thermo Scientific, San Jose, California, United States of America;3Quest Diagnostics – Nichols Institute, San Juan Capistrano, California, United States of America |
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| Abstract: | Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use. |
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