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Isolation and Sequencing of a Genomic Clone for Mouse Brain Specific Small RNA |
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| Authors: | Kaijiro Anzai Shunsuke Kobayashi Narumi Kitamura Yuri Kanai Hiromichi Nakajima Yoshioki Suehiro Sataro Goto |
| Institution: | Department of Biochemistry, School of Pharmaceutical Science, Toho University, Miyama, Funabashi, Chiba, Japan |
| Abstract: | We isolated a mouse genomic clone that hybridized with small RNA present in the cytoplasm of the brain. The RNA was about 150 nucleotides long. This RNA seemed to be specific to the brain, since it was not found in the liver or kidney. The clone DNA contained a sequence homologous to 82-nucleotide "identifier" core sequence of cDNA clones of rat. The sequence contained a split promoter for RNA polymerase III and was flanked by a 12-nucleotide direct repeat (ATAAATAATTTA). |
| Keywords: | Mouse genomic clone Brain specific small RNA Northern blot analysis Dot blot hybridization Nucleotide sequence analysis |